PDF | Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted. PDF | Gerbera jamesonii (gerbera) is an important cut-flower in the global floricultural industry. Micropropagation is the main system used to. PDF | In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L-1 BAP and mg .

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Propagation Ornamental Plants, 4: Pharmacological studies on folk medicine in Sichuan province, China.

The studing of micropropagation of Gerbera sp via shoot tip explant.

Many temperate and tropical plants have been successfully propagated via tissue culture. Capitulum explant promoted callus development. The regenerated plants were observed to be normal. Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog MS medium supplemented with 2.

Percentage of callus and root formation per explants produced on MS micropropgaation supplemented with different hormones and concentration after 8 weeks in culture formed in these experiments were mainly green.

The most important aspect in plant tissue micropropagstion is the capability of cultured cells and tissues to regenerate into complete plants. The study was conducted at laboratory B2. Micropropagation of Saintpaulia ionantha was optimized when petiole explants were cultured on MS medium supplemented with 2.


Gerbera jamesonii is also used in the preparation of traditional Chinese medicine, tu-er-feng, for curing cold and also for treating rheumatism Ye et al. A potent cytokinin for woody plant tissue culture.

Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort. Finally the seeds were rinsed 3 times with sterile distilled water. In vitro establishment and plantlet regeneration of Gerbera jamesonii Bolus ex.

Percentage of shoot formation and number of shoots per explants produced on MS medium supplemented with different hormones and concentrations after 8 weeks in culture. Explants were obtained from 8-week-old aseptic seedlings. High concentrations of cytokinin in culture medium were found to be unsuitable for shoot formation from leaf or petiole explants in some ornamentals.


The present study showed that only petiole explant produced optimum results for shoot formation. Different types of auxin and cytokinin combinations were used in order to obtain complete regeneration of Gerbera in vitro.

MICROPROPAGATION OF GERBERA | ICAR-Indian Institute of Horticultural Research

Takayama and Misawa reported that medium containing 0. Plantlets were transferred to soil and maintained in culture room for weeks for adaptation process before being transferred to field environment. Tissues of explants generally show distinct planes of cell division, various specializations of cells and organization into specialized structures such as microprropagation vascular system. Percentage of callus and root formation per explants produced on MS medium supplemented with different hormones and concentration after 8 weeks in culture.

Bigot reported that the addition of g L -1 activated charcoal in the culture medium showed vigorous rooting from excised shoots of Begoniax hiemalis. Kajian morfogenesis tumbuhan hiasan Saintpaulia ionantha Wendl. Pakistan Journal of Biological Sciences, The authors wish to thank the Institute of Biological Sciences and University of Malaya, Kuala Lumpur, Malaysia for providing all facilities and financial support.

It is reported that a good micropropagatiln of cytokinin and auxin in the culture medium enhanced good shoot formation and plantlet regeneration from explants, for example, adventitious Gerbera shoots were regenerated from flower buds of greenhouse grown plants Pierik et al. Plantlets survival rate achieved was Because genetic variability within the Gerbera genus is relatively limited, breeding potential for new flower colours and patterns as well as resistance to biotic or abiotic stresses is also limited Orlikowska et al.

Regenerated plants were rooted on Murashige and Skoog basal medium. Percentage of shoot formation and number of shoots per explants produced on MS medium supplemented with different hormones and concentrations after 8 weeks in culture supplemented with 2.

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Plant Cell Tissue Organ Culture, Gerbera plantlets formed were transferred to soil with The physiological status of the donor is determined by environmental conditions such as temperature, light intensity, day-length and light wavelength. Plant hormones and type of explants play very important roles in determining mifropropagation of Gerbera jamesonii in vitro.

However, white, creamy friable callus was obtained when explants were cultured on MS medium supplemented with 2, 4-D alone.

Micropropagation is the main system used to clonally propagate gerbera in vitro resulting in the production of millions of plantlets each year. In the present study, propagation of Gerbera jamesonii through tissue culture techniques was done and the factors influencing the growth of this plant were studied.

Direct organogenesis from mature leaf and petiole explants of Eryngium foetidum L. In vitro plantlet production from young capitulum explants of Gerbera jamesonii. The mode of action of thidiazuron: Combination of auxin and cytokinin induces the formation of adventitious shoots gerbrea roots.

In vitro Culture of Higher Plants. The lowest shoot formation was observed when explants were cultured on MS medium supplemented with 0. In Gerbera jamesoniiBAP was also required for the formation of callus. Formation of callus from explants tissues involves the development of progressively more random planes of cell division, less frequent specialization of cells and loss of organized structures Thorpe, ; Wagley et al.

Organogenesis and histogenesis of adventitious organ induced on leaf blade segments of Begonia elatior hybrids Begonia x hiemalis in tissue culture. Micropropagation selected somaclones Begonia Saintpaulia J. Survival rate of micro propagated plants that were transferred to soil were investigated Fig.

Cytokinin alone miceopropagation the culture medium induces shoot formation in many plants.

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